WVU Researcher Investigates ‘Biofilms’ In Water Pipes

For most of us, we turn on the water faucet and clean water comes out. But we may not realize the water pipes that deliver the water to our homes have a micro slime inside them.

For most of us, we turn on the water faucet and clean water comes out. But we may not realize the water pipes that deliver the water to our homes have a micro slime inside them. 

WVU professor and researcher Emily Garner has a grant from the National Science Foundation to look into micro-organisms in water systems. She spoke with News Director Eric Douglas to explain what she is finding. 

This interview has been lightly edited for clarity. 

Douglas: First off, introduce yourself and explain who you are. 

Garner: My name is Emily Garner. I’m an assistant professor in Civil and Environmental Engineering at West Virginia University. I’ve been in that position for four years and I study the role of microorganisms and engineered systems for the treatment and transport of drinking water and wastewater.

Douglas: Let’s talk for just a second about what it takes to deliver water from the treatment plant to your house.

Garner: When drinking water leaves the treatment plant, it still has a really long journey to travel before it arrives at your home or the businesses in your community. It can take days or even weeks for the water to make that journey. It’s one of the biggest jobs that our water utilities have – is making sure that that water stays high quality and safe for people to drink from the time it leaves the treatment plant to the time when it arrives at people’s homes. Even a relatively small community might have hundreds of miles of pipe that are buried underground.

Douglas: That’s, that’s actually kind of stunning. I would never have thought it would be in the system that long. Is it going to a holding tank somewhere?

Garner: Certainly tanks are really prevalent throughout distribution systems, especially in West Virginia, where we’ve got a lot of hills. They can help us overcome some of the elevation differences that might exist throughout a community. Those things are also really important for holding water so the treatment plant can treat the same amount of water throughout the day and night and not need to kind of adjust to the fact that everyone wakes up at 6am and takes a shower. But water doesn’t usually sit in those tanks for days or weeks at a time. It’s just that when water has to travel through hundreds of miles of pipe, it can take a really long time. That time just starts to add up.

Douglas: Explain to me what biofilms are within the water distribution system.

Garner: It’s very normal in any aquatic environment. If you’ve gone down to the river or the stream, you might see kind of a film that forms on the surface of rocks. That’s exactly what we’re talking about in drinking water. But of course, the water is much cleaner. When that water leaves the treatment plant, the utility has dosed some sort of chlorine disinfectant to kill harmful microorganisms. But there’s lots of research out there that shows that there still might be some microorganisms present in that water. Most of those are going to be harmless, they’re not going to make people sick but as that water flows over the surface of pipes continuously, it can lead to formation of those biofilms. 

We care about those for a number of different reasons. When they accumulate in great enough quantities, they can affect water quality in different ways like compounds that affect taste and odor. They could slough off into the water and lead to discoloration events. Really importantly, they can also create environments where harmful bacteria do get into the system. While these biofilms are totally normal, in small quantities, it is really important to have strategies to control them and to make sure they don’t get out of hand and start to accumulate in ways that can affect water quality. 

Douglas: You’re not talking like big green slimy build up inside of a water pipe. This is a microscopic level, typically.

Garner: We’re talking about these really, really thin biofilms. But when they accumulate on the inside of many, many miles of pipe, it can still be something that can affect water quality.

Douglas: In West Virginia, especially in the rural areas, some of the smaller communities, there’s some aging infrastructure, there’s aging water systems. So what do we do about some of that? Is that a growing problem? 

Garner: Certainly our infrastructure is aging across the country, but certainly in a lot of parts of West Virginia. I think it’s important to be really concerned about the state of that buried infrastructure that we can’t see that was maybe put in the ground 50, 60 plus years ago. And so that’s absolutely an important thing to be concerned with, and making sure that we can minimize some of these impacts to water quality. 

Douglas: Is chlorine what we’re using and it just works best beyond anything? 

Garner: It’s a balancing act. Chlorine is really, really important. You know, it wasn’t much more than 100 years ago that we had diseases like cholera that were affecting huge swaths of the population because we weren’t able to disinfect our water before we drank it. So chlorine is absolutely essential, making sure we can disinfect that water is absolutely essential. 

But today, we do know that it can react with other compounds in water, like organic matter, to create compounds known as disinfection byproducts. A lot of these disinfection byproducts are possible carcinogens. And so we certainly want to minimize how prevalent those are in our water. It’s a really big balancing act for water utilities to deal with: how do they make sure there’s enough chlorine present in our water to kill microorganisms, while making sure that they don’t contribute to the propagation of these disinfection byproducts? And that’s one of the reasons we really care about control of biofilms. Because organic matter can accumulate in those biofilms — microorganisms are organic, they create organic compounds, to help them kind of stick to the walls of the pipe. And so controlling biofilms are also important to help make that balancing act a little bit easier.

Douglas: One of the big issues facing the water community is people who can work in these systems, who have a lot of these water facilities, are aging out or they’re retiring. Talk to me a little bit about what you’re doing to help get people who can work in the water systems?

Garner: This grant from the National Science Foundation that is supporting a lot of my work, it has two major goals. One is research, and the other is education. That education includes things like, I plan to work with a lot of undergraduate and graduate students so they will come out of this better trained to engineer good systems, designing good systems that can address some of these challenges that we’re talking about. 

But the other part of my education component associated with this grant is through K through 12 outreach. My goal is to help K through 12 students better understand what opportunities they might have for careers in the water sector. I want them to, you know, decide whether or not they want to pursue careers in that field, how important water workers in our state are for the health of our communities.

Douglas: What haven’t we talked about?

Garner: I did want to mention that for this National Science Foundation project, one of our main goals for this research is to better integrate our understanding of the microbiology of drinking water systems with modeling of flow patterns present in drinking water distribution systems. With lots of other aquatic environments, we know that the forces that are exerted by flowing water can impact how biofilms grow, but we don’t really have a thorough understanding of how flow impacts what happens to microorganisms in drinking water distribution systems. And part of why this is so important, and interesting to my research team, is that one of our key hypotheses driving this research is that we think these conditions will be very different in rural areas where it can take many, many miles of pipe to reach even a relatively few number of homes in a small community compared to much more densely populated urban areas where we’ve got a lot more data on this subject. 

That’s what one of our goals is, to better understand what some of the challenges that might exist to integrating flow modeling of distribution systems with understanding microbiology especially in rural communities.

WVU Engineering Program Gets $599K Boost

The project involves examining different learning techniques in engineering curricula to improve student performance and improve retention rates in associated academic fields.

On Thursday, U.S. Sen. Joe Manchin announced $599,999 for an engineering education research project at West Virginia University. The funding comes from a grant from the National Science Foundation. 

The project involves examining different learning techniques in engineering curricula to improve student performance and improve retention rates in associated academic fields.

Manchin said the engineering education research project will bolster academic success and help retain students in the engineering disciplines.  

The U.S. National Science Foundation is an independent federal agency that promotes science and engineering in all 50 US states and territories. 

Investments account for around 25 percent of federal support to colleges like WVU for basic research.

Marshall University To Help Unlock The Secrets Of Water Bears

The National Science Foundation awarded Marshall University a $366,624 grant to study tardigrades in a partnership with the University of North Carolina at Chapel Hill.

The National Science Foundation awarded Marshall University a $366,624 grant to study how protective structures adopted by tardigrades help protect them from their environment. The study will be part of a partnership with the University of North Carolina at Chapel Hill.

Tardigrades, also known as water bears or moss piglets, are micro-animals that are found in almost every place on earth. They are known for being able to survive the harshest environments.

“Tardigrades produce these compounds known as free radicals, which are reactive chemical species, when they’re exposed to stressors,” said Derrick Kolling, chair of the Marshall University Department of Chemistry.

You can see responses to extreme conditions in nature, but the reason why tardigrades are unique is their vast variety of responses to environmental stressors such as vacuums, UV radiation, dehydration, high salt concentration, and freezing temperatures. A common response by tardigrades includes forming into a tun.

“Forming this structure called a tun, they sort-of shrink down; extrude water, and then they can stay in that state for a very long period of time,” Kolling said.

West Virginia Public Broadcasting
When a tardigrade is stressed, it enters a quasi-death state. The animal retracts its head and its eight legs and curls into a dried-up ball.

According to Kolling, looking into these chemical structures could help development in space travel and pharmaceutical storage. They might even help fight against aging.

“Throughout our lives, we develop damage in our bodies,” Kolling said. “Not that we’re necessarily going to form one of these protective states, but these things might give us insight into how humans might protect our DNA.”

In a partnership with Robert C. Byrd Institute, the research team is starting the Tardigrade Trading Post outreach program. The trading post will send scientific kits designed by the research team to be used by citizen researchers. This includes students K-12; what Kolling referred to as little citizen scientists.

The Robert C. Byrd Institute is designing 3D printed microscopes that citizen researchers can use to gather tardigrades from their backyards. On top of providing young students an opportunity to learn about micro-species, Kolling hopes that this will help the team harvest a wide variety of tardigrade species.

“A lot of water bear species are parthenogenic, which means that you only need the female to reproduce so they can reproduce asexually,” Kolling said. “I think the odds of finding a new species are pretty decent, especially if people are willing to go to places like Dolly Sods. We could raise them in the lab. If we find interesting conditions, we would characterize them genetically.”

The grant will support the graduate and undergraduate student research team for the next three years.

Marshall University
Derrick Kolling, chair of the Marshall University Department of Chemistry

National Science Foundation Awards $880,000 For W.Va. Projects

The National Science Foundation has awarded more than $880,000 for projects at West Virginia University and Fairmont State University.

The awards total $889,849 and were announced Tuesday by the state’s U.S. senators, Democrat Joe Manchin and Republican Shelley Moore Capito.

The individual awards include $749,693 to Fairmont State for a project to support low-income undergraduate students in science, technology, engineering and math disciplines; $90,156 to West Virginia University for a project to enhance understanding, modeling and forecasting of variations in the upper atmosphere; and $50,000 to WVU to develop a health care patient scheduling system to predict patient no-shows.

WVU Researchers Work To Improve Access To Blue Economy

Two West Virginia University researchers are in Phase One of a study funded by the National Science Foundation. They are working to find ways to connect underserved populations to natural environments, specifically the ocean, through what is called the Blue Economy. Ross Andrew and Robert Burns spoke with Eric Douglas to explain the project.

This interview has been lightly edited for clarity.

Douglas: Give me the elevator version of what this project is about.

Andrew: I think ultimately, the goal is to bring together different disciplines to address what we see as a weakness in the blue economy. So for example, we have people that are experts in social science, like Robert, who understand visitor dynamics, studying people, as they do recreation or travel. We have people that are economists that are really focused on the dollars and cents. We have people that study race, and are really focusing their scholarship on how different races and ethnicities sort of experience natural resources. And then we have folks from the business realm that are hoping to ultimately take this research product, this process that we’re going through, and make it something that can be functional in society moving forward to sort of create that benefit.

Douglas: What is the blue economy?

Burns: I think the best definition is going to be all of those sources of benefit, really, that come from ocean and coastal resources, at least through the lens of the US. But the blue economy is anything that you can connect to those ocean and coastal resources. And it’s not necessarily just the products that are tangible, like eating seafood, or shipping things from here to Europe or here to Asia. It’s also those, those industries that exist because of the ocean.

Douglas: Why the focus on people of color and the blue economy?

Burns: For 50 years, we’ve studied Caucasian use in blue and green environments — ocean and land environments. And I’ve been a part of that for 20-some years. Every survey that we do, every time we survey people, we get about three to seven percent people of color in our database for that particular study. In this case, we’ve flipped the survey methodology where all of the people that we’re going to interview and work with are people of color, whether they’re African American, Hispanic, or Indigenous or Asian.

Douglas: Let’s walk through that process. Where does it go? What are the downstream efforts?

Andrew: Basically, we’re being funded right now to address this problem. We want to produce a product that goes out to people. And we’re trying to treat this like a business startup. At the end of phase one, we’re going to have some sort of low fidelity prototype that we can then give to people in different areas that are connected to the blue economy and these resources and say, “Use this. Try this. Does this app, does this website, does this data-structuring tool that we’ve made to be user-friendly work?”

It’s not a dusty report that everybody has to read through. It’s not a whole matrix of ones and zeros of data. But it’s something that we can use, maybe it’s almost like a game. But it basically tells that story of connection, and allows people to explore these resources a little more effectively than what they get now.

Douglas: For West Virginians historically, it may not be true as much today, but there’s always been that connection between West Virginia and Myrtle Beach. How does this apply to West Virginians who would go to Myrtle Beach?

Burns: I think the real answer is that the study that we’re conducting in the Florida Keys and in the Great Lakes is designed to be replicated and extended elsewhere. We want Myrtle Beach to be as accessible to underserved communities as the Florida Keys. We know that in this area, West Virginia is the heart of Appalachia. There are a lot of underserved populations, a lot of people who don’t have access to go to Florida Keys, but they can drive to Myrtle Beach.

Part of the process is informing people, for example, in West Virginia, of the opportunities that exist so they can develop those place attachments with those areas and make sure that they’re accessible for the income that exists in West Virginia. There are a lot of high-end opportunities in Myrtle Beach, and there are the kinds of opportunities for folks who don’t have a lot of funds. So we want to be able to provide people with more opportunities, including those from West Virginia.

If the researchers are approved for Phase Two next year, they will be eligible to receive a $5 million grant to continue their work. The grant is funded through the National Science Foundation and the National Oceanic and Atmospheric Administration.

WVU Professor Wins $500,000 to Work on Cybersecurity

A West Virginia University professor has won a $500,000 grant to work on cybersecurity techniques.

The college announced Monday that Yanfang Ye has won the CAREER award from the National Science Foundation.

Ye is an assistant professor of computer science and electrical engineering at WVU. Her plan is to design a way to better analyze cybercrime so it’s easier to spot illegal behavior and piece together online criminal networks.

Her grant will be doled out over the next five years.

The college says Ye’s work has won numerous awards and brought in nearly $3 million in federal research funding in the past two years.

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