Norfolk Southern reached a deal on Wednesday with the Brotherhood of Maintenance of Way employees to provide up to seven days a year of paid sick leave.
The deal follows another with CSX in recent weeks. Norfolk Southern is the second-largest carrier in West Virginia behind CSX, with about 800 miles of track.
Like CSX, Norfolk Southern is a major transporter of coal from Appalachia to eastern ports.
The Norfolk Southern deal covers about 3,000 employees who repair and maintain the track.
The lack of paid sick leave was a major issue last year when several rail unions threatened a nationwide strike.
In December, Congress approved a labor agreement between the railroads and the White House, but it did not include paid sick leave.
Earlier this week, western carrier Union Pacific reached a deal on paid sick leave with two unions.